Laboratory Testing Cutoffs & Methods

The following is an explanation of RTL's urine screening and confirmation procedures/cutoff levels. The routine cutoff levels listed below may periodically change.

Screen Reporting Levels and Methods
Confirmation Cutoff Levels and Methods

Comprehensive Panel Screen

The following list provides screening detail for the Comprehensive Drug Test panel. RTL screens urine specimens by enzyme assay (EA), enzyme immunoassay (EIA), and liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Screen Reporting Levels By Procedure

Drug	Method	Cutoff
Alcohol (Ethanol)	EA	0.04 g/dL
Amphetamines - Amphetamines - Methylenedioxyamphetamine (MDA) - Methylenedioxymethamphetamine (MDMA) - Methylenedioxyethylamphetamine (MDEA)	EIA LC-MS/MS LC-MS/MS LC-MS/MS	1000 ng/mL 250 ng/mL 250 ng/mL 250 ng/mL
Anticonvulsants - Carbamazepine and/or Metabolite - Oxcarbazepine Metabolite	LC-MS/MS LC-MS/MS	100 ng/mL 100 ng/mL
Antidepressants - Amitriptyline/Nortriptyline - Bupropion Metabolite - Citalopram/Escitalopram - Desipramine/Imipramine - Doxepin - Fluoxetine and/or Metabolite - Maprotiline - Paroxetine - Sertraline - Trazodone/Nefazodone and/or Metabolite - Venlafaxine Metabolite	LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS	100 ng/mL 250 ng/mL 150 ng/mL 100 ng/mL 100 ng/mL 150 ng/mL 100 ng/mL 150 ng/mL 150 ng/mL Qualitative 100 ng/mL
Barbiturates	EIA	200 ng/mL
Benzodiazepines - Alprazolam Metabolite - Clonazepam Metabolite - Flunitrazepam Metabolite - Flurazepam Metabolite - Lorazepam - Midazolam Metabolite - Nordiazepam - Oxazepam - Temazepam - Triazolam Metabolite	LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS	50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL
Cocaine	EIA	300 ng/mL
Methylphenidate	LC-MS/MS	50 ng/mL
Narcotics/Opiates - Buprenorphine - Butorphanol Metabolite - Codeine - Fentanyl - Hydrocodone - Hydromorphone - Ketamine Metabolite - Meperidine Metabolite - Methadone - Morphine - Nalbuphine - Oxycodone/Oxymorphone - Pentazocine - Propoxyphene - Tramadol and/or Metabolite	EIA LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS EIA LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS EIA LC-MS/MS	5 ng/mL Qualitative 100 ng/mL 5 ng/mL 100 ng/mL 100 ng/mL 25 ng/mL 25 ng/mL 150 ng/mL 100 ng/mL 100 ng/mL 50 ng/mL 100 ng/mL 300 ng/mL 100 ng/mL
Phencyclidine (PCP)	EIA	25 ng/mL
Sedative/Hypnotic Agents - Carisoprodol/Meprobamate - Zolpidem Metabolite	LC-MS/MS LC-MS/MS	200 ng/mL 10 ng/mL
THC (Marijuana)	EIA	50 ng/mL

Comprehensive Panel Confirmation

The following list provides confirmation detail for the Comprehensive Drug Test panel. RTL confirms urine specimens by gas chromatography-mass spectrometry (GC-MS), liquid chromatography- tandem mass spectrometry (LC-MS/MS) and/or gas chromatography- flame ionization detector (GC-FID).

Confirmation Cutoff Levels By Procedure

Drug	Method	Cutoff
Alcohol (Ethanol)	GC-FID	0.02 g/dL
Amphetamines - Amphetamine - Methamphetamine - Methylenedioxyamphetamine (MDA) - Methylenedioxymethamphetamine (MDMA) - Methylenedioxyethylamphetamine (MDEA)	LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS	250 ng/mL 250 ng/mL 250 ng/mL 250 ng/mL 250 ng/mL
Anticonvulsants - Carbamazepine - 10,11-Dihydroxycarbazepine - Oxcarbazepine - 10-Hydroxycarbazepine	GC-MS GC-MS GC-MS GC-MS	100 ng/mL Qualitative 100 ng/mL 100 ng/mL
Antidepressants - Amitriptyline - Nortriptyline - Hydroxybupropion - Citalopram/Escitalopram - Desipramine - Imipramine - Doxepin - Desmethyldoxepin - Fluoxetine - Norfluoxetine - Maprotiline - Paroxetine - Sertraline - Norsertraline - mCPP - Hydroxy-mCPP - Trazodone - Hydroxytrazodone - Venlafaxine - O-Desmethylvenlafaxine	LC-MS/MS LC-MS/MS GC-MS GC-MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS GC-MS GC-MS GC-MS GC-MS GC-MS GC-MS GC-MS GC-MS GC-MS GC-MS LC-MS/MS LC-MS/MS	25 ng/mL 25 ng/mL 250 ng/mL 150 ng/mL 25 ng/mL 25 ng/mL 25 ng/mL 25 ng/mL 150 ng/mL 150 ng/mL 100 ng/mL 150 ng/mL 150 ng/mL 150 ng/mL 100 ng/mL Qualitative Qualitative Qualitative 100 ng/mL 100 ng/mL
Barbiturates - Amobarbital - Butabarbital - Butalbital - Pentobarbital - Phenobarbital - Secobarbital	GC-MS GC-MS GC-MS GC-MS GC-MS GC-MS	200 ng/mL 200 ng/mL 200 ng/mL 200 ng/mL 200 ng/mL 200 ng/mL
Benzodiazepines - alpha-Hydroxyalprazolam (Alprazolam) - 7-Aminoclonazepam (Clonazepam) - 7-Aminoflunitrazepam (Flunitrazepam) - 2-Hydroxyethyl flurazepam (Flurazepam) - Lorazepam - alpha-Hydroxymidazolam (Midazolam) - Nordiazepam - Oxazepam - Temazepam - alpha-Hydroxytriazolam (Triazolam)	LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS	50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL
Cocaine	LC-MS/MS	100 ng/mL
Methylphenidate	GC-MS	Qualitative
Narcotics/Opiates - Buprenorphine - Norbuprenorphine - Butorphanol - Hydroxybutorphanol - Codeine - Fentanyl - Hydrocodone - Hydromorphone - Ketamine - Norketamine - Dehydronorketamine - Normeperidine - Methadone - Methadone Metabolite (EDDP) - Morphine - Nalbuphine - Noroxycodone - Oxycodone - Oxymorphone - Pentazocine - Propoxyphene - Norpropoxyphene - Tramadol - O-Desmethyl-cis-Tramadol	LC-MS/MS LC-MS/MS GC-MS GC-MS LC-MS/MS GC-MS LC-MS/MS LC-MS/MS GC-MS GC-MS GC-MS GC-MS LC-MS/MS LC-MS/MS LC-MS/MS GC-MS LC-MS/MS LC-MS/MS LC-MS/MS GC-MS GC-MS GC-MS LC-MS/MS LC-MS/MS	0.5 ng/mL 2 ng/mL 5 ng/mL Qualitative 100 ng/mL 5 ng/mL 100 ng/mL 100 ng/mL 5 ng/mL 5 ng/mL 25 ng/mL 50 ng/mL 100 ng/ml 100 ng/mL 100 ng/mL 250 ng/mL 50 ng/mL 50 ng/mL 50 ng/mL 250 ng/mL 200 ng/mL 200 ng/mL 100 ng/mL 100 ng/mL
Phencyclidine (PCP)	GC-MS	5 ng/mL
Sedative/Hypnotic Agents - Carisoprodol - Meprobamate - Zolpidem - Carboxyzolpidem	LC-MS/MS LC-MS/MS LC-MS/MS LC-MS/MS	100 ng/mL 100 ng/mL 1 ng/mL 10 ng/mL
THC (Marijuana) - THC-COOH (Marijuana) - THC-COOH/Creatinine Ratio	LC-MS/MS Calculation	5 ng/mL

Urinalysis Adulteration Examination

RTL's Standard Operating Procedures (SOP's) require that each urine specimen be examined for signs of adulteration. This examination includes the following

Physical Examination: Color, excessive foaming, excess sediments, or unusual odor are noted. If adulteration is suspected, this is noted on the final report.
Analytical Examination: Upon request, pH, creatinine (tested by default) and specific gravity are run to aid in determining possible adulteration.

To learn more about RTL's laboratory drug tests call:

Laboratory Testing Cutoffs & Methods

Comprehensive Panel Screen

Screen Reporting Levels By Procedure

Comprehensive Panel Confirmation

Confirmation Cutoff Levels By Procedure

Urinalysis Adulteration Examination

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